关键词: 急性肺损伤, 炎性因子, 大鼠

Abstract: Objective To investigate the effect of Shegan Zhike Capsule on lipopolysaccharide ( LPS) - induced acute lung injury (ALI) in rats. Method 70 rats were randomly divided into 7 groups according to their body weights, namely, blank group, model group, low, medium and high dose groups of Shegan Zhike capsule (30. 00 mg, 60. 00 mg,120. 00 mg extract / kg) ,Zhongyang and Xiyang groups, with 10 rats in each group. They were administered for 7 consecutive days. On the fifth day of the experiment, 1 h after the administration by gavage, except for the blank group, rats in other groups were instilled with LPS (5 mg / kg) intratracheally to make Ali rat models, The blank control group was infused with the same dose of normal saline. 48 hours after model establishment, blood was collected from abdominal aorta of rats to measure IL-1β、TNF-α content. The whole lung was taken and weighed to calculate the lung index. The left lung tissue of rats was taken to measure the wet / dry mass ratio of the lung. The right middle lobe of lung was taken for pathological observation. Result Compared with the model control group, The contents of IL-1β and TNF-αin the low, medium and high dose groups ( 30. 00 mg, 60. 00 mg, 120. 00 mg extract / kg) of Shegan Zhike capsule were significantly decreased (P<0. 01) ; Compared with the model control group, the lung index and lung wet / dry mass ratio of rats in the high dose group of Shegan Zhike capsule were significantly reduced ( P< 0. 01,P< 0. 05) ;Compared with the blank control group, the alveolar space of the model control group was widened, the alveolar cavity exudation, and the degree of alveolar damage and destruction were significantly increased ( P<0. 01,P<0. 05) . Conclusion Shegan Zhike capsule has a protective effect on lipopolysaccharide induced acute lung injury in rats, and its mechanism may be related to reducing pulmonary edema and inhibiting the synthesis and release of inflammatory factors.

Key words: acute lung injury, inflammatory factors, rat